Journal: Food & Nutrition Research

Article Title: Chronic liquid fructose supplementation does not cause liver tumorigenesis but elicits clear sex differences in the metabolic response in Sprague–Dawley rats

doi: 10.29219/fnr.v65.7670

Figure Lengend Snippet: Biochemical parameters of Sprague–Dawley rats corresponding to the different study groups (CT, control; FRC, fructose; F+RVT, fructose plus resveratrol), segregated by rat sex. Plasma uric acid (A), leptin (B), adiponectin (C), triglyceride (E), non-esterified fatty acids (NEFA) (F) and liver triglyceride (D) concentrations. a P < 0.001, b P < 0.01 versus the corresponding male rat group; *** P < 0.001, ** P < 0.01, * P < 0.05 versus its respective sex control; # P < 0.05, ## P < 0.01, ### P < 0.001 versus fructose of the same sex.

Article Snippet: Non-esterified fatty acid (NEFA) colorimetric kit was obtained from BiooScientific (Austin, TX, USA) and Uric acid kit from SpinReact (Girona, Spain) (Ref. no. 1001010).

Techniques:

Journal: PLoS Neglected Tropical Diseases

Article Title: High Fat Diet Modulates Trypanosoma cruzi Infection Associated Myocarditis

doi: 10.1371/journal.pntd.0003118

Figure Lengend Snippet: ( a ) Both RD and HFD fed infected mice display a significant (p≤0.005) increase in serum TG content at 15 dpi which decrease significantly by the end of acute infection (30 dpi) compared with their respective uninfected groups as quantified by colorimetric analysis. However, serum TG content of uninfected RD fed mice was higher (38%) than uninfected HFD fed mice (n = 5). Results are presented as % difference compared to uninfected RD fed mice at day 0 post infection(d0 pi = 100%). ( b ) Colorimetric analysis of free fatty acids (FFA) demonstrated a significant (p≤0.005) increase in serum FFA content at 15 dpi which decrease significantly by the end of acute infection (30 dpi) compared with their respective uninfected groups (n = 5). Results are presented as % difference compared to uninfected RD fed mice at day 0 post infection(d0 pi = 100%). ( c ) Analysis of total cholesterol (TC) levels in the RD fed mice showed a significant decrease (p≤0.005) even at 15 dpi compared to uninfected mice (n = 5). The uninfected and infected HFD fed mice showed higher levels of TC at 15 dpi compared to uninfected RD and infected RD fed mice respectively, (n = 5, p≤0.005). However at d30pi, both the HFD and RD fed infected mice displayed significantly reduced serum TC levels (p≤0.005). Results are presented as % difference compared to uninfected RD fed mice at day 0 post infection(d0 pi = 100%). ( d ) Acute infection results in elevated LDL/cholesterol levels in heart and WAT. Colorimetric analysis demonstrated a significant (p≤0.005) increase in the cholesterol levels in the hearts (260%) and the WATs (394%) of the infected RD fed mice compared to uninfected RD fed mice (100%) (n = 5). Though infection increased cholesterol levels in the HFD fed infected mice, the analysis demonstrated a significant decrease in their level in both the hearts (202%) and WATs (211%) compared to uninfected RD fed control mice (n = 5). Results are presented as % difference compared to uninfected RD fed mice (100%) (p≤0.005). Bars represent mean values of the data with SEM as vertical lines. Significant change is represented by black stars compared with uninfected RD mice. Significant difference between infected RD and infected HFD is represented by white stars. (p<0.005).

Article Snippet: Colorimetric assays were performed using colorimetric assay kit for non-esterified fatty acid (NEFA from Cell Biolabs, Inc. CA), triglyceride (TG from Cayman Chemicals, MI), and low density lipoprotein (LDL), high density lipoprotein (HDL) and total cholesterol (TC) (Enzychrom (E2HL-100), Bioassay system, CA) in serum samples 6 .

Techniques: Infection, Control